Evaluación del efecto de promotores de maduración oocitaria in vitro en bovinos
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Universidad de La Salle. Facultad de Ciencias Agropecuarias. Medicina Veterinaria
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El objetivo de esta investigación fue comparar la influencia de los suplementos en los medios de maduración oocitaria y su posterior fertilización. Los suplementos probados fueron el suero fetal bovino (SFB), el suero de vaca en estro(SVE), el suero de neonato precalostral (SNP) Y el suero de yegua en estro(SYE). Los oocitos fueron obtenidos de ovarios de vacas de abasto publico. Los ovarios fueron trasportados en solución salina a una temperatura de 37 grados centígrados. Una vez llegaban los ovarios al laboratorio fueron lavados en hipoclorito y por NaCl 0.9% durante 5 minutos y posteriormente aspirados. Lo oocitos encontrando fueron depositados en los diferentes medios de maduración en los cuales se depositaron n=108 oocitos por cada tratamiento. Una vez madurados los oocitos fueron depositados en medio de fecundación por 18 horas. Pasadas las 18 horas de fecundación los oocitos fueron pasados al medio de maduración de embriones el cual constaba de PBS+SFB 10%. Al sexto día los embriones obtenidos eran observados al microscopio y se realizaba el respectivo conteo. El presente estudio fue analizado por la prueba estadística de CHI2 con el cual se comprobó que si que existió diferencia significativa entre los diferentes sueros con respecto a la maduración y fertilización de los oocitos.
The objective of this investigation was to compare the influence of the supplements in the means of oocitarian maturation and the later fertilization. The proven supplements were the bovine fetal serum (BFS), the cow serum in estrous (CSE), the newborn precalostral serum (NPS) and the mare serum in estrous (MSE). The oocytes were obtained from ovariums of public supply cows. The ovariums were carried in saline solution with a temperature of 37 degrees Celsius. Once the ovariums arrived to the laboratory they were washed in hypochlorite and sodium chloride 0.9% during five minutes and after that aspirated. The opposing oocitos were deposited in the different maturation means in which n=108 oocitos were deposited by each treatment. Once the oocitos were matured they were deposited in fecundation means by 18 hours. After the 18 hours of fecundation the oocytes were passed to the means of maturation of embryos which consisted of PBS+SFB 10%. To the sixth day the obtained embryos were observed to the microscope and carried out to the respective count. This study was analyzed by stadistic proof of CHI2 . With which was proven that if significant difference existed among the different serums with regard to the maturation and fertilization of the oocitos.
The objective of this investigation was to compare the influence of the supplements in the means of oocitarian maturation and the later fertilization. The proven supplements were the bovine fetal serum (BFS), the cow serum in estrous (CSE), the newborn precalostral serum (NPS) and the mare serum in estrous (MSE). The oocytes were obtained from ovariums of public supply cows. The ovariums were carried in saline solution with a temperature of 37 degrees Celsius. Once the ovariums arrived to the laboratory they were washed in hypochlorite and sodium chloride 0.9% during five minutes and after that aspirated. The opposing oocitos were deposited in the different maturation means in which n=108 oocitos were deposited by each treatment. Once the oocitos were matured they were deposited in fecundation means by 18 hours. After the 18 hours of fecundation the oocytes were passed to the means of maturation of embryos which consisted of PBS+SFB 10%. To the sixth day the obtained embryos were observed to the microscope and carried out to the respective count. This study was analyzed by stadistic proof of CHI2 . With which was proven that if significant difference existed among the different serums with regard to the maturation and fertilization of the oocitos.
Palabras clave
Ganado bovino, Inseminación artificial, Embriología animal, Reproducción