Diagnóstico de Leptospirosis canina por medio de la técnica Dot - Elisa en perros con enfermedad renal en la ciudad de Bogotá
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Universidad de La Salle. Facultad de Ciencias Agropecuarias. Medicina Veterinaria
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Se estudiaron treinta caninos con enfermedad renal a los cuales se les realizó pruebas de diagnóstico serológico para Leptospirosis por medio de la prueba de Aglutinación Microscópica (MAT) a seis serovares de Leptospira interrogans; y pruebas de diagnóstico en muestra de orina por medio de las pruebas de campo oscuro, intento de aislamiento y Dot – ELISA a los serovares canicola, icterohaemorrhagiae, pomona y grippotyphosa. Se diagnóstico como positivos a Leptospira como etiología de la enfermedad renal uniendo los resultados de las cuatro pruebas a: 10 caninos (33.3%) que presentaron títulos a MAT a los serovares (sv) icterohaemorrhagiae, canicola y grippotyphosa principalmente; de estos, 9 fueron positivos a Dot – ELISA con una distribución homogénea en los cuatro serovares que se manejaron sv icterohaemorrhagiae, sv canicola, sv pomona y sv grippotyphosa; 3 perros fueron positivos a la prueba de campo oscuro y ninguno resultó positivo al aislamiento. De los 20 perros restantes (66.7%) dos quedaron con diagnóstico no confirmado de Leptospirosis pues quedaron sospechosos en el campo oscuro y en el aislamiento. La asociación entre la prueba Dot – ELISA y la prueba MAT fue altamente significativa (P
Thirty dogs with renal diseases were studied. Serologic diagnostic test for Leptospirosis was realized them by a Microscopy Agglutination Test (MAT) at six serovars of Leptospira interrogans; and diagnostic tests in urine sample by Darkfield Microscopy, Organism Isolation and Dot – ELISA at serovars canicola, icterohaemorrhagiae, pomona y grippotyphosa. As a cause of the renal disease, the study found positive results in the Leptospira Diagnostic, taking in account the 4 tests, in the 30 animals: 10 dogs (33.3%) which presented titers on MAT mainly in the following serovars (sv) icterohaemorrhagiae, canicola y grippotyphosa. Dot – ELISA was positive in 9 of the cases with homogeneous distribution in the serovars sv icterohaemorrhagiae, sv canicola, sv pomona y sv grippotyphosa; 3 dogs had positive results in the Darkfield Microscopy Test and neither of these 10 cases obtained positive results in the Isolation Test. The rest of the animals (20 cases, 66.7%) had a negative Leptospirosis Diagnostic although 2 of these cases did not have confirmed Leptospirosis diagnostic because the tests of Darkfield microscopy and Isolation were suspicious. The association between the Dot – ELISA test and MAT test was highly significative (P
Thirty dogs with renal diseases were studied. Serologic diagnostic test for Leptospirosis was realized them by a Microscopy Agglutination Test (MAT) at six serovars of Leptospira interrogans; and diagnostic tests in urine sample by Darkfield Microscopy, Organism Isolation and Dot – ELISA at serovars canicola, icterohaemorrhagiae, pomona y grippotyphosa. As a cause of the renal disease, the study found positive results in the Leptospira Diagnostic, taking in account the 4 tests, in the 30 animals: 10 dogs (33.3%) which presented titers on MAT mainly in the following serovars (sv) icterohaemorrhagiae, canicola y grippotyphosa. Dot – ELISA was positive in 9 of the cases with homogeneous distribution in the serovars sv icterohaemorrhagiae, sv canicola, sv pomona y sv grippotyphosa; 3 dogs had positive results in the Darkfield Microscopy Test and neither of these 10 cases obtained positive results in the Isolation Test. The rest of the animals (20 cases, 66.7%) had a negative Leptospirosis Diagnostic although 2 of these cases did not have confirmed Leptospirosis diagnostic because the tests of Darkfield microscopy and Isolation were suspicious. The association between the Dot – ELISA test and MAT test was highly significative (P
Palabras clave
Caninos, Enfermedades leptospirosis canina, Técnica de Elisa, Perros