Evaluación de la viabilidad y el desarrollo de embriones bovinos obtenidos por fertilización in vitro incubados en oviductos ovinos
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2010
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Universidad de La Salle. Facultad de Ciencias Agropecuarias. Medicina Veterinaria
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Este estudio pretendía evaluar la utilización de biomodelos en el desarrollo embrionario teniendo en cuenta la viabilidad y calidad de los mismos. Se produjeron 214 embriones bovinos en el laboratorio a partir de 1052 oocitos aspirados de 74 ovarios provenientes de hembras bovinas sacrificadas en planta de sacrificio. Los embriones fueron divididos en 5 grupos en partes iguales, un grupo se desarrollaron totalmente en el laboratorio, y los restantes se incubaron hasta el día 6 en oviductos de ovinos criollos de lana. El día 6 se procedió a recuperar los embriones por medio de la técnica quirúrgica y ha ser evaluados y comparados en cuanto a calidad basados en la morfología con el grupo de laboratorio. Se concluyo que la técnica de incubación in vivo de embriones bovinos en oviductos ovinos permite el desarrollo de embriones de altísima calidad. El grupo de embriones producidos totalmente en el laboratorio alcanzo un número mayor de estructuras grado 1 frente a los embriones incubados in vivo, 18 (40%) y 3 (8.8%) respectivamente. Los embriones incubados en oviductos ovinos alcanzaron mayor número de estructuras grado 2 que los producidos totalmente en el laboratorio, 3 (8.8%) y 1 (2.2%) respectivamente.
This study aimed to evaluate the use of biomodels in embryonic development, taking into account feasibility and quality of embryos. 214 bovine embryos were produced in the laboratory from 1052 oocytes of 74 ovaries aspirated from female cattle slaughtered in the slaughter plant. The embryos were divided into 5 equal groups, one group fully developed in the laboratory, and the remaining were incubated until day 6 in oviducts of native sheep. Day 6 is sought to recover the embryos through the surgical technique has to be evaluated and compared in terms of quality based on morphology with the lab group. It was concluded that the technique of in vivo incubation of bovine embryos in sheep oviducts allows the development of high quality embryos. The group of embryos produced entirely in the laboratory reached a larger number of structures grade 1 compared to embryos incubated in vivo, 18 (40%) and 3 (8.8%) respectively. The embryos incubated in sheep oviducts reached greater number of structures grade 2 that the laboratory group, 3 (8.8%) and 1 (2.2%) respectively.
This study aimed to evaluate the use of biomodels in embryonic development, taking into account feasibility and quality of embryos. 214 bovine embryos were produced in the laboratory from 1052 oocytes of 74 ovaries aspirated from female cattle slaughtered in the slaughter plant. The embryos were divided into 5 equal groups, one group fully developed in the laboratory, and the remaining were incubated until day 6 in oviducts of native sheep. Day 6 is sought to recover the embryos through the surgical technique has to be evaluated and compared in terms of quality based on morphology with the lab group. It was concluded that the technique of in vivo incubation of bovine embryos in sheep oviducts allows the development of high quality embryos. The group of embryos produced entirely in the laboratory reached a larger number of structures grade 1 compared to embryos incubated in vivo, 18 (40%) and 3 (8.8%) respectively. The embryos incubated in sheep oviducts reached greater number of structures grade 2 that the laboratory group, 3 (8.8%) and 1 (2.2%) respectively.
Palabras clave
Reproducción de ganado bovino, Embriología veterinaria, Cultivo in vitro, Embriones