Determinación de la viabilidad espermática post-descongelamiento, bajo efecto de la adición fraccionada de dimetilformamida en caballo criollo colombiano
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2015
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Universidad de La Salle. Facultad de Ciencias Agropecuarias. Medicina Veterinaria
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El objetivo de este trabajo fue evaluar el efecto de la adición fraccionada del crioprotector Dimetilformamida y la viabilidad espermática pre y post descongelamiento. Se utilizaron seis sementales de la raza criollo colombiano. La dimetilformamida se añadió de la siguiente manera: T1: adición de una décima parte del crioprotector por minuto; T2: adición de una décima parte del crioprotector cada dos minutos y T3: adición de una décima parte del crioprotector cada tres minutos. Para los tratamientos, el semen se mantuvo diluido y a temperatura ambiente, durante un periodo medio de una hora y diez minutos. Posteriormente las muestras se enfriaron a 5ºC utilizando una tasa de enfriamiento de 0,25ºC / min. Las curvas se realizaron de la siguiente forma: C1: Curva de enfriamiento de 0,25 ° C a 5 ° C, curva de descongelación rápida que va directamente al vapor de nitrógeno, C2: Curva de enfriamiento de 0,25 ° C a 5 ° C, temperatura de equilibrio por 45 minutos, curva lenta en congelador y C3: Curva de enfriamiento 0,25 ° C a 5 ° C, temperatura de equilibrio por 45 minutos, curva rápida en vapor de nitrógeno. Después de congelar las muestras, se sumergieron en nitrógeno líquido. La descongelación se realizó a 52ºC durante diez segundos, se sumergieron las muestras en agua a temperatura de 37ºC bajo un tiempo de treinta segundos. Inmediatamente después se evaluó la Motilidad total, motilidad progresiva y vigor espermático, bajo microscopía óptica (40x). En el análisis estadístico no se observaron diferencias estadísticamente significativas (P
The aim of this study was to evaluate the effect of the fractional addition of cryoprotectant dimethylformamide and sperm viability pre and post thawing. Six stallions were used race Colombian Creole. Dimethylformamide was added as follows: T1: adding one tenth of cryoprotectant per minute; T2: adding a cryoprotectant tenth every two minutes T3: adding a cryoprotectant tenth every three minutes. For treatments, diluted semen was kept at room temperature for an average period of one hour and ten minutes. Subsequently the samples were cooled to 5 ° C using a cooling rate of 0.25 ° C / min. The curves were performed as follows: C1: 0.25ºC cooling curve to 5 ° C, rapid Thawing curve that goes directly to the nitrogen vapor, C2: cooling curve of 0.25 ° C to 5 ° C, equilibrium temperature for 45 minutes in freezer and slow curve C3: 0.25 ºC Cooling curve to 5 ° C, the equilibrium temperature for 45 minutes, quick nitrogen vapor curve. After freezing the samples were immersed in liquid nitrogen. Thawing was performed at 52º C for ten seconds, the samples were immersed in water at 37° C under a thirty second time. Immediately after total motility, sperm motility and vigor under optical microscopy (40x) was evaluated. In the statistical analysis, no statistically significant differences (P
The aim of this study was to evaluate the effect of the fractional addition of cryoprotectant dimethylformamide and sperm viability pre and post thawing. Six stallions were used race Colombian Creole. Dimethylformamide was added as follows: T1: adding one tenth of cryoprotectant per minute; T2: adding a cryoprotectant tenth every two minutes T3: adding a cryoprotectant tenth every three minutes. For treatments, diluted semen was kept at room temperature for an average period of one hour and ten minutes. Subsequently the samples were cooled to 5 ° C using a cooling rate of 0.25 ° C / min. The curves were performed as follows: C1: 0.25ºC cooling curve to 5 ° C, rapid Thawing curve that goes directly to the nitrogen vapor, C2: cooling curve of 0.25 ° C to 5 ° C, equilibrium temperature for 45 minutes in freezer and slow curve C3: 0.25 ºC Cooling curve to 5 ° C, the equilibrium temperature for 45 minutes, quick nitrogen vapor curve. After freezing the samples were immersed in liquid nitrogen. Thawing was performed at 52º C for ten seconds, the samples were immersed in water at 37° C under a thirty second time. Immediately after total motility, sperm motility and vigor under optical microscopy (40x) was evaluated. In the statistical analysis, no statistically significant differences (P
Palabras clave
Espermatogenesis en animales, Semen congelado, Caballos criollos, Inseminación artificial, Cría de caballos