Distribución biológica del polímero ROD3-C14, y sus reacciones tisulares en perros, como contribución al control de la incontinencia urinaria humana
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2003
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Universidad de La Salle. Facultad de Ciencias Agropecuarias. Medicina Veterinaria
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Como una alternativa de tratamiento para la incontinencia urinaria por dilatación en humanos, se planteó la utilización de inóculos artificiales que ayuden a la vejiga a cerrar su cuello disminuyendo la presión que ejerce la orina sobre el esfínter. De esta forma se desarrolla un producto que para el efecto se llamará ROD3 el cual debe ser inoculado en el cuello de la vejiga; con el fin de determinar si permanece o no en el lugar de inoculación, se marcará con Carbono 14 por lo que se llamará ROD3 - C14, éste le enviará ondas radiactivas que serán leídas por placas de autoradiografía, es decir, que si se inocula en la vejiga no debe aparecer en ningún órgano diferente a ese. El procedimiento se llevó a cabo 60 y 120 días después de inoculado con cinco caninos hembras para cada caso, posteriormente se expusieron las placas radiográficas a los seis órganos principales de cada animal durante 120 días. Por otra parte para garantizar que el producto permanecía en su sitio, se analizó el proceso histológico que se llevó a cabo a su alrededor con el fin de encontrar un encapsulamiento de tejido fibroso o conectivo, para ello se analizaron las muestras 60 y 120 días post - inoculo tanto macroscópica como microscópicamente. La migración del producto ROD3 - C14 no pudo ser expuesta por medio de la marcación a las placas, ya que solo se obtuvieron en el revelado manchas dispersas en las placas, sin embargo la ubicación de los órganos tampoco coincidió con la presentación de las marcaciones, lo que lleva a concluir que hubo errores en los procedimientos. En cuanto a la determinación de encapsulamiento, se encontró una gruesa capa de tejido conectivo firme que rodeaba el producto en 16 de las 20 inoculaciones (dos en cada perra, una subcutánea y otra periuretral), las cuatro otras no fueron encontradas, y no se observaron ninguna diferencia de forma, tamaño o consistencia tanto micro como macroscópicamente, entre el grupo No. 1 (60 días de inoculado) y el grupo No. 2 (120 días de inoculado). Esto lleva a concluir que el producto permanece firme en el sitio de inoculación y es separado en cápsula por el mismo organismo sin producir reacciones nocivas. 1 (60 días de inoculado) y el grupo No. 2 (120 días de inoculado). Esto lleva a concluir que el producto permanece firme en el sitio de inoculación y es separado en cápsula por el mismo organismo sin producir reacciones nocivas. 1 (60 días de inoculado) y el grupo No. 2 (120 días de inoculado). Esto lleva a concluir que el producto permanece firme en el sitio de inoculación y es separado en cápsula por el mismo organismo sin producir reacciones nocivas.
As an alternative for the treatment of urinary incontinence by dilatation in humans, the inoculation of an artificial substance was proposed to help the bladder to close its neck and to diminish the pressure of the urine on the sphincter. So a product named ROD3 was developed, to be inoculated in the neck of the bladder. In order to determine if it remained on the site of inoculation, it was marked with Carbon 14, thus called ROD3 - C 14, that should allow it to emit radioactive waves that can be readed by auto radiographic plaques, which means that if it's inoculated in the bladder, it should not appear in any different organ but the bladder. The procedure was made 60 and 120 days after the inoculation in 5 bitches for each case. Afterwards the radiographic plaques were exposed on the 6 principal organs of each animal for 120 days. On the other side, to assure that the product remained on its site, the histological process that took place around it, was analyzed in order to find a capsule of fibrotic or connective tissue. Therefore the samples were examinated 60 and 120 days post inoculation in a macro and microscopic way. The marking of the plaques could not expose the migration of the ROD3 - C14 product because in the revelation process the unique results were dispersing stains all over the plaques. However the location of the organs did not match either with the presentation of the marks, which leads to conclude that mistakes were made in the procedure. In the determination of the capsule formation, a thick layer of connective tissue was found, that surrounded the product in 16 of the 20 inoculations (2 in each bitch, 1 subcutaneous and 1 periuretral). The other four were not found and no difference was observed in shape, size or consistence either in micro- or macroscopic way between the group number 1 (with 60 days of inoculation) and the group number 2 (witht 120 days of inoculation). This leads to conclude that the product remains firmly on the site of inoculation and that it is separated in a capsule by the same organism without causing any noxious reactions.
As an alternative for the treatment of urinary incontinence by dilatation in humans, the inoculation of an artificial substance was proposed to help the bladder to close its neck and to diminish the pressure of the urine on the sphincter. So a product named ROD3 was developed, to be inoculated in the neck of the bladder. In order to determine if it remained on the site of inoculation, it was marked with Carbon 14, thus called ROD3 - C 14, that should allow it to emit radioactive waves that can be readed by auto radiographic plaques, which means that if it's inoculated in the bladder, it should not appear in any different organ but the bladder. The procedure was made 60 and 120 days after the inoculation in 5 bitches for each case. Afterwards the radiographic plaques were exposed on the 6 principal organs of each animal for 120 days. On the other side, to assure that the product remained on its site, the histological process that took place around it, was analyzed in order to find a capsule of fibrotic or connective tissue. Therefore the samples were examinated 60 and 120 days post inoculation in a macro and microscopic way. The marking of the plaques could not expose the migration of the ROD3 - C14 product because in the revelation process the unique results were dispersing stains all over the plaques. However the location of the organs did not match either with the presentation of the marks, which leads to conclude that mistakes were made in the procedure. In the determination of the capsule formation, a thick layer of connective tissue was found, that surrounded the product in 16 of the 20 inoculations (2 in each bitch, 1 subcutaneous and 1 periuretral). The other four were not found and no difference was observed in shape, size or consistence either in micro- or macroscopic way between the group number 1 (with 60 days of inoculation) and the group number 2 (witht 120 days of inoculation). This leads to conclude that the product remains firmly on the site of inoculation and that it is separated in a capsule by the same organism without causing any noxious reactions.
Palabras clave
Radioactividad, Fibrosis, Isotopos, Enfermedades urinarias