Evaluación de la sustitución de cloroformo en la purificación de PHB producido por medio de la bacteria Bacillus megaterium en un medio de reacción a partir de cascarilla de arroz
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2024
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La producción desmedida de plástico sintético y las malas prácticas de desechos aplicadas a subproductos provenientes de la industria alimentaria como la cascarilla de arroz, aportan a la contaminación de fuentes hídricas y de ecosistemas. Ante esta problemática, la producción y aplicación de bioplásticos obtenidos con subproductos de la industria alimentaria (como los polihidroxialcanoatos, entre ellos el PHB), son una solución viable para reducir los niveles de contaminación aportados por los factores mencionados, sin embargo, estas prácticas necesitan de propuestas que permitan mayor accesibilidad y menor impacto ambiental para su producción, por lo que la presente investigación busca exponer al propionato de isoamilo como posible solvente efectivo en el proceso de recuperación de biomasa de polihidroxialcanoatos, contenida en el microorganismo Bacillus megaterium. Se aplica una metodología dividida en 3 fases, la primera dedicada a la producción de un hidrolizado de cascarilla de arroz mediante pretratamientos físicos e hidrolisis ácida, la segunda enfocada a la producción de PHB por de la bacteria B.megaterium usando el hidrolizado de cascarilla de arroz como fuente de carbono y la tercera a la comparación del PHB recuperado por propionato de isoamilo con relación al recuperado con cloroformo.
The excessive production of synthetic plastic and poor waste practices applied to by-products from the food industry such as rice husk, contribute to the contamination of water sources and ecosystems. Faced with this problem, the production and application of bioplastics obtained from by-products of the food industry (such as polyhydroxyalkanoates, including PHB), are a viable solution to reduce the levels of contamination contributed by the aforementioned factors. However, these practices require proposals that allow greater accessibility and less environmental impact for their production, which is why the present research seeks to expose isoamyl propionate as a possible effective solvent in the process of recovering polyhydroxyalkanoate biomass, contained in the microorganism Bacillus megaterium. A methodology divided into three phases is applied, the first dedicated to the production of a rice husk hydrolysate by physical pretreatments and acid hydrolysis, the second focused on the production of PHB by the bacteria B. megaterium using rice husk hydrolysate as a carbon source and the third to the comparison of the PHB recovered by isoamyl propionate in relation to that recovered with chloroform.
The excessive production of synthetic plastic and poor waste practices applied to by-products from the food industry such as rice husk, contribute to the contamination of water sources and ecosystems. Faced with this problem, the production and application of bioplastics obtained from by-products of the food industry (such as polyhydroxyalkanoates, including PHB), are a viable solution to reduce the levels of contamination contributed by the aforementioned factors. However, these practices require proposals that allow greater accessibility and less environmental impact for their production, which is why the present research seeks to expose isoamyl propionate as a possible effective solvent in the process of recovering polyhydroxyalkanoate biomass, contained in the microorganism Bacillus megaterium. A methodology divided into three phases is applied, the first dedicated to the production of a rice husk hydrolysate by physical pretreatments and acid hydrolysis, the second focused on the production of PHB by the bacteria B. megaterium using rice husk hydrolysate as a carbon source and the third to the comparison of the PHB recovered by isoamyl propionate in relation to that recovered with chloroform.
Palabras clave
Protección del grano, Factores ambientales, Material ignífugo, Bacillus megateriu, Industria alimentaria, Cascarilla de arroz