Title

Genotype identification of Staphylococcus with methicillin-resistant phenotype isolated from human, animal and environmental samples

DOI

https://doi.org/10.15381/rivep.v30i1.14614

Document Type

Article

Publication Date

1-1-2019

Publication Title

Revista de Investigaciones Veterinarias del Peru

Abstract

The aim of this study was to identify the mecA-1 gene of Staphylococcus isolates with methicillin-resistant phenotype (SMR) obtained from biological samples and surfaces of a veterinary clinic. Nine isolates with SMR phenotype classified by the automated VITEK system were selected. The genus and the variability were corroborated by amplification and sequencing of the 16S rRNA and tuf genes. The presence of the mecA-1 gene was also established by PCR. In the nine isolates with SMR phenotype, the 16S rRNA and tuf genes were identified, showing 99% similarity with reference strains; however, only in five isolates was found the mecA-1 gene. The results showed a greater identification of the MR genotype in intra-hospital surfaces, which indicates the need for control and monitoring of these strains in veterinary hospitals due to the implications they have on public health.

Volume

30

Issue

1

First Page

364

Last Page

376

ISSN

16823419

Identifier

SCOPUS_ID:85065616848

Documento original

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