Title
Comparative assessment of the replication efficiency of dengue, yellow fever, and chikungunya arboviruses in some insect and mammalian cell lines
DOI
https://doi.org/10.1590/0037-8682-0511-2018
Document Type
Article
Publication Date
1-1-2019
Publication Title
Revista da Sociedade Brasileira de Medicina Tropical
Abstract
Introduction: Insect cell cultures play an essential role in understanding arboviral replication. However, the replicative efficiency of some of these viruses such as dengue (DENV), yellow fever (YFV), and chikungunya (CHIKV) in a new cellular substrate (Lulo) and in the other two recognized cell lines has not been comparatively assessed. Methods: Vero, C6/36, and Lulo cell lines were infected with DENV, YFV, and CHIKV. The viral progeny was quantified through plaque assays and quantitative reverse transcription-polymerase chain reaction, while for DENV2, the findings were confirmed by immunofluorescence antibody assay. Results: The higher DENV2 titer (from multiplicity of infection 0.001) was obtained on day four post-infection in C6/36 and on day six in Vero cells, while the Lulo cell line was almost impossible to infect under the same conditions. However, C6/36 showed the highest values of viral RNA production compared to Vero cells, while the quantification of the viral RNA in Lulo cells showed high levels of viral genomes, which had no correlation to the infectious viral particles. Conclusions: C6/36 was the most efficient cell line in the alpha and flavivirus production, followed by Vero cells. Thus, Lulo cells may be a useful substrate to study the mechanisms by which cells evade viral replication.
Volume
52
ISSN
00378682
Recommended Citation
Segura Guerrero, Nidya Alexandra and Bello García, Felio Jesús, "Comparative assessment of the replication efficiency of dengue, yellow fever, and chikungunya arboviruses in some insect and mammalian cell lines" (2019). Scopus Unisalle. 189.
https://ciencia.lasalle.edu.co/scopus_unisalle/189
PubMed ID
31038623
Identifier
SCOPUS_ID:85065471511