Title
Cryopreservation method and composition of the vitrification solution affect viability of in vitro bovine embryos
DOI
https://doi.org/10.17533/udea.rccp.v29n2a06
Document Type
Article
Publication Date
4-1-2016
Publication Title
Revista Colombiana de Ciencias Pecuarias
Abstract
Background: An optimal formulation for vitrifying in vitro-produced (IVP) bovine embryos is currently unavailable. Objective: To estimate whether differences in composition of vitrification solutions may affect the viability of IVP embryos as compared to that of embryos cryopreserved by a conventional slow-freezing method. Methods: Bovine IVP embryos were cryopreserved by two methods: 1) a slow controlled-rate (1.5 M ethylene glycol (EG)), or 2) vitrification by using two different vitrification and thawing/warming solutions: (1) Protocol V1: Commercial vitrification Kit, and (2) Protocol V2: Defined vitrification (20% EG; 20% dimethyl sulfoxide (DMSO); 20% fetal bovine serum (FBS)) and warming (20% FBS; 0.2 M sucrose) solutions. Embryo viability was recorded at 24, 48, and 72 h after thawing/warming by evaluating the number of embryos that re-expanded and developed to the hatching blastocyst stage. Results: Embryo survival rate was affected by the method of cryopreservation, where the frequency of embryos that re-expanded at 24 h after thawing/warming was higher for embryos vitrified with protocols V1 and V2 (89.0%, 86.2%, respectively) compared to those cryopreserved by the slow-controlled rate method (73.6%, p<0.05). Similarly, higher percentage of embryos cryopreserved by vitrification hatched at 72 h, where protocol V2 resulted in higher percentage of hatched embryos (84.3%) compared to protocol V1 (64,0%, p<0.05), and both were higher compared to the slow-controlled rate method (55.2%, p<0.05). Conclusions: The method of cryopreservation and composition of the vitrification solution have a direct effect on the viability of bovine IVP embryos.
Volume
29
Issue
2
First Page
130
Last Page
137
ISSN
01200690
Recommended Citation
Vargas Reyes, José N. and Chacón Jaramillo, Liliana, "Cryopreservation method and composition of the vitrification solution affect viability of in vitro bovine embryos" (2016). Scopus Unisalle. 371.
https://ciencia.lasalle.edu.co/scopus_unisalle/371
Identifier
SCOPUS_ID:84966737777