Title
Study of primary cell cultures derived from lucilia sericata (Diptera: Calliphoridae)
Document Type
Article
Publication Date
1-1-2009
Publication Title
Revista Ciencias de la Salud
Abstract
The main purpose of this study was to obtain primary cell cultures derived from Lucilia sericata (Diptera: Calliphoridae). Necrophagous this fly is used for determination of post-mortem interval and larval therapy. Since explants embryonated eggs were performed in various culture media (Grace Schneider, MM/VP12, DMEM, Grace/L-15 and L-15), supplemented with 20% fetal serum. Sterilization of the biological material was carried out by immersing it in formaldehyde and sodium hypochlorite solutions. The cell growth was initiated in the L-15, MM/VP12, and Schneider Grace/L-15 in an average time of 10 days after completion of planting by the proliferation of groups of colonies scattered on the surface of the boxes crops and also from the endings of larval fragments. The evolution of cell growth to the formation of monolayer semi-confluent was relatively fast, reaching at 3 weeks post-explant. Cellular morphology in cultured cells was heterogeneous, especially epithelioid forms, similar to nerve, giant and irregular. Comparison of the growth characteristics of these cell cultures with those obtained from other species of flies was more favorable in the evolution of those obtained from L. sericata, on the grounds that the cells are better adapted to the physical-chemical conditions of several culture media. This is the first report of a cell culture-fly family Calliphoridae.
Volume
7
Issue
3
First Page
63
Last Page
74
ISSN
16927273
Recommended Citation
Echeverry Prieto, Lena Carolina; Zapata Lesmes, Ángela Cristina; Segura, Alexandra; and Bello, Felio Jesús, "Study of primary cell cultures derived from lucilia sericata (Diptera: Calliphoridae)" (2009). Scopus Unisalle. 675.
https://ciencia.lasalle.edu.co/scopus_unisalle/675
Identifier
SCOPUS_ID:77649141866