Title
Virulence characteristics of Escherichia coli isolates obtained from broiler breeders with salpingitis
DOI
https://doi.org/10.1016/j.cimid.2004.03.001
Document Type
Article
Publication Date
1-1-2005
Publication Title
Comparative Immunology, Microbiology and Infectious Diseases
Abstract
Thirty isolates of Escherichia coli from broiler breeders with salpingitis were studied. Using the slide agglutination test, the isolates were found to belong to serogroups O1, O2, O5, O36, O45, O53 and O78. Pathogenicity for day-old chicks was determined by air sac inoculation and isolates were categorized as having high, intermediate or low virulence. Growth on iron starvation medium was observed together with aerobactin production. Based on the results of in vitro adherence tests, attachment to oviduct epithelium from old birds was found to be superior to that observed using corresponding material from young birds. DNA hybridization testing for type 1, P, and S fimbriae revealed predominant expression of type 1, correlating with mannose-sensitive hemagglutination using guinea-pig erythrocytes. In this study, P and S fimbriae were not considered to be important adherence factors. Study findings would suggest that, as far as salpingitis is concerned, type 1 fimbriae can play an important role in E. coli infection in breeders. An interesting result to emerge from the study was the observation that E. coli isolates were completely resistant to serum from young breeders, whereas they were completely sensitive using serum from older breeders. Based on serogroups involved, pathogenicity for day-old chicks and virulence indicators, the salpingitis isolates were similar to those from cases of chronic respiratory disease. © 2004 Elsevier Ltd. All rights reserved.
Volume
28
Issue
1
First Page
1
Last Page
15
ISSN
01479571
Recommended Citation
Monroy, Maria A.R.; Knöbl, Terezinha; Bottino, José A.; Astolfi Ferreira, Claudete S.; and Piantino Ferreira, Antonio J., "Virulence characteristics of Escherichia coli isolates obtained from broiler breeders with salpingitis" (2005). Scopus Unisalle. 725.
https://ciencia.lasalle.edu.co/scopus_unisalle/725
PubMed ID
15563950
Identifier
SCOPUS_ID:10244235404