A New Continuous Cell Line from the Mosquito Psorophora confinnis (Diptera: Culicidae) and its Susceptibility to Infections with Some Arboviruses
DOI
https://doi.org/10.1590/S0074-02762001000600022
Document Type
Article
Publication Date
1-1-2001
Publication Title
Memorias do Instituto Oswaldo Cruz
Abstract
A new cell line, PC-0199-BR, was established from embryonated eggs of the mosquito Psorophora confinnis. To date (September 2000) it has had 62 continuous passages. This is the first report of a cell line of mosquitoes belonging to the genus Psorophora. Cell growth initially was achieved in the MM/ VP12 medium, supplemented with 20% fetal bovine serum; however, the subcultures were later adapted to Grace's medium with 10% fetal bovine serum. Cell morphology in the primary cultures was heterogeneous; but later in the established cell line, the predominant cell type was epithelioid. Cultured cells were predominantly diploid (2n=6); however, chromosome abnormalities were observed in a small proportion of the cells in later passages. C and G band patterns were also determined in the karyotype. The cell line isozyme profiles coincided with pupae and adult samples of the species taken from the same colony. A preliminary arbovirus susceptibility study for the cell line was undertaken. No evidence was observed of contamination of the cell line with bacteria, fungi or mycoplasma.
Volume
96
Issue
6
First Page
865
Last Page
873
ISSN
00740276
Recommended Citation
Bello, Felio Jesús; Rodríguez, Jaime A.; Escovar, Jesús; Olano, Víctor A.; Morales, Alberto; González, Martha; and Rey, Gloria, "A New Continuous Cell Line from the Mosquito Psorophora confinnis (Diptera: Culicidae) and its Susceptibility to Infections with Some Arboviruses" (2001). Scopus Unisalle. 736.
https://ciencia.lasalle.edu.co/scopus_unisalle/736
PubMed ID
11562717
Identifier
SCOPUS_ID:0042410615