Title
Establishment and Characterization of a New Continuous Cell Line from Lutzomyia longipalpis (Diptera: Psychodidae) and its Susceptibility to Infections with Arboviruses and Leishmania chagasi
DOI
https://doi.org/10.1590/S0074-02762000000100017
Document Type
Article
Publication Date
1-1-2000
Publication Title
Memorias do Instituto Oswaldo Cruz
Abstract
Embryonic tissue explants of the sand fly Lutzomyia longipalpis (Lutz & Neiva 1912) the main vector of Leishmania chagasi (Cunha and Chagas), were used to obtain a continuous cell line (Lulo). The tissues were seeded in MM/VP12 medium and these were incubated at 28°C. The first subculture was obtained 45 days after explanting and 96 passages have been made to date. Lulo is composed of epithelioid cells, showed a 0.04 generations/hour exponential growth rate and population doubling time at 24.7 h. The cell line isoenzymatic profiles were determined by using PGI, PGM, MPI and 6-PGDH systems, coinciding with patterns obtained from the same species and colony s pupae and adults. The species karyotype characteristics were recognized (2n = 8), in which pair 1 is subtelocentric and pairs 2, 3 and 4 are metacentric. Lulo was free from bacterial, fungal, mycoplasmic and viral infection. Susceptibility to five arbovirus was determined, the same as Lulo interaction with Leishmania promastigotes.
Volume
95
Issue
1-2
First Page
103
Last Page
110
ISSN
00740276
Recommended Citation
Rey, Gloria J.; Ferro, Cristina; and Bello, Felio Jesús, "Establishment and Characterization of a New Continuous Cell Line from Lutzomyia longipalpis (Diptera: Psychodidae) and its Susceptibility to Infections with Arboviruses and Leishmania chagasi" (2000). Scopus Unisalle. 739.
https://ciencia.lasalle.edu.co/scopus_unisalle/739
PubMed ID
10656714
Identifier
SCOPUS_ID:0033630633