Title

Establishment and Characterization of a Cell Line from the Mosquito Anopheles albimanus (Diptera: Culicidae)

DOI

https://doi.org/10.1590/S0074-02761997000100027

Document Type

Article

Publication Date

1-1-1997

Publication Title

Memorias do Instituto Oswaldo Cruz

Abstract

A new cell line designated LSB-AA 695BB, was established from embryos of the mosquito Anopheles albimanus. The primary culture was initiated in April, 1995, and the first passage was made 48 days later. Serial subcultures of the cells have been carried through 90 passages from Abril 1995 to February 1996. The cells were grown at 28°C in MK/VP12 medium, supplemented with 20% fetal bovine serum; the pH tolerance ranged between 6.8 to 7.0. The cells have also been adapted to MM/VP12 medium under the same pH, temperature and serum concentration. The majority of the cells were a fibroblast-type Isozyme characterization showed a pattern similar to that of An. albimanus pupae and adults but distinct from Ae. taeniorhynchus and Ae. albopictus (C6/36) mosquito cell lines. The culture was shown to be free of mycoplasma, bacteria and fungi. Microsporidia contamination of transovarial transmission was controlled with 6.0 μg/ml of albendazole.

Volume

92

Issue

1

First Page

123

Last Page

128

ISSN

00740276

PubMed ID

9302423

Identifier

SCOPUS_ID:0030621945

Documento original

Compartir

COinS