Title

Evaluating the anti-leishmania activity of Lucilia sericata and Sarconesiopsis magellanica blowfly larval excretions/secretions in an in vitro model

DOI

https://doi.org/10.1016/j.actatropica.2017.09.033

Document Type

Article

Publication Date

1-1-2018

Publication Title

Acta Tropica

Abstract

Leishmaniasis is a vector-borne disease caused by infection by parasites from the genus Leishmania. Clinical manifestations can be visceral or cutaneous, the latter mainly being chronic ulcers. This work was aimed at evaluating Calliphoridae Lucilia sericata- and Sarconesiopsis magellanica-derived larval excretions and secretions' (ES) in vitro anti-leishmanial activity against Leishmania panamensis. Different larval-ES concentrations from both blowfly species were tested against either L. panamensis promastigotes or intracellular amastigotes using U937-macrophages as host cells. The Alamar Blue method was used for assessing parasite half maximal inhibitory concentration (IC ) and macrophage cytotoxicity (LC ). The effect of larval-ES on L. panamensis intracellular parasite forms was evaluated by calculating the percentage of infected macrophages, parasite load and toxicity. L. sericata–derived larval-ES L. panamensis macrophage LC was 72.57 μg/mL (65.35–80.58 μg/mL) and promastigote IC was 41.44 μg/mL (38.57–44.52 μg/mL), compared to 34.93 μg/mL (31.65–38.55 μg/mL) LC and 23.42 μg/mL (22.48–24.39 μg/mL) IC for S. magellanica. Microscope evaluation of intracellular parasite forms showed that treatment with 10 μg/mL L. sericata ES and 5 μg/mL S. magellanica ES led to a decrease in the percentage of infected macrophages and the amount of intracellular amastigotes. This study produced in vitro evidence of the antileishmanial activity of larval ES from both blowfly species on different parasitic stages and showed that the parasite was more susceptible to the ES than it's host cells. The antileishmanial effect on L. panamensis was more evident from S. magellanica ES. 50 50 50 50 50 50

Volume

177

First Page

44

Last Page

50

ISSN

0001706X

PubMed ID

28982577

Identifier

SCOPUS_ID:85030679884

Documento original

Compartir

COinS