Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived lulo cell line
DOI
https://doi.org/10.1590/0074-02760200113
Document Type
Article
Publication Date
9-1-2020
Publication Title
Memorias do Instituto Oswaldo Cruz
Abstract
Lutzomyia longipalpis-derived cell line (Lulo), has been suggested as a model for studies of interaction between sand flies and Leishmania. Here, we present data of proteomic and gene expression analyses of Lulo cell related to interactions with L. (V.) braziliensis. Lulo cell protein extracts were analysed through a combination of two-dimensional gel electrophoresis and mass spectrometry and resulting spots were further investigated in silico to identify proteins, using Mascot search, and, afterwards, resulting sequences were applied for analysis with VectorBase. Sixty-four spots were identified showing similarities to other proteins registered in the databases and could be classified according to their biological function, such as ion-binding proteins (23 %), proteases (14 %), cytoskeletal proteins (11 %) and interactive membrane proteins (9.5 %). Effects of interaction with L. (V.) braziliensis with the expression of three genes (enolase, tubulin, and vacuolar transport protein) were observed after an 8-hour timeframe and compared to culture without parasites, and demonstrated the impact of parasite interaction with the expression of the following genes: LLOJ000219 (1.69-fold), LLOJ000326 (1.43-fold) and LLOJ006663 (2.41-fold). This set of results adds relevant information regarding the usefulness of the Lulo cell line for studies with Leishmania parasites that indicate variations of protein expression.
Volume
115
Issue
9
First Page
1
Last Page
23
ISSN
00740276
Recommended Citation
Monteiro de Côrtes, Luzia; de Pita-Pereira, Daniela; Grijó Farani, Priscila Silva; Santini Pereira, Bernardo Acácio; Dias Lopes, Geovane; Souza da Silva, Franklin; Rsende Corrêa, Paloma; Macedo Silva, Roger Magno; Côrte-Real, Suzana; Bello, Felio Jesús; Mendonça-Lima, Leila; da Cruz Moreira, Otacilio; Caldas Waghabi, Mariana; and Alves, Carlos Roberto, "Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived lulo cell line" (2020). Scopus Unisalle. 60.
https://ciencia.lasalle.edu.co/scopus_unisalle/60
PubMed ID
33111757
Identifier
SCOPUS_ID:85092369861