Title

Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived lulo cell line

DOI

https://doi.org/10.1590/0074-02760200113

Document Type

Article

Publication Date

9-1-2020

Publication Title

Memorias do Instituto Oswaldo Cruz

Abstract

Lutzomyia longipalpis-derived cell line (Lulo), has been suggested as a model for studies of interaction between sand flies and Leishmania. Here, we present data of proteomic and gene expression analyses of Lulo cell related to interactions with L. (V.) braziliensis. Lulo cell protein extracts were analysed through a combination of two-dimensional gel electrophoresis and mass spectrometry and resulting spots were further investigated in silico to identify proteins, using Mascot search, and, afterwards, resulting sequences were applied for analysis with VectorBase. Sixty-four spots were identified showing similarities to other proteins registered in the databases and could be classified according to their biological function, such as ion-binding proteins (23 %), proteases (14 %), cytoskeletal proteins (11 %) and interactive membrane proteins (9.5 %). Effects of interaction with L. (V.) braziliensis with the expression of three genes (enolase, tubulin, and vacuolar transport protein) were observed after an 8-hour timeframe and compared to culture without parasites, and demonstrated the impact of parasite interaction with the expression of the following genes: LLOJ000219 (1.69-fold), LLOJ000326 (1.43-fold) and LLOJ006663 (2.41-fold). This set of results adds relevant information regarding the usefulness of the Lulo cell line for studies with Leishmania parasites that indicate variations of protein expression.

Volume

115

Issue

9

First Page

1

Last Page

23

ISSN

00740276

PubMed ID

33111757

Identifier

SCOPUS_ID:85092369861

Documento original

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